{"id":6862,"date":"2023-10-10T11:09:29","date_gmt":"2023-10-10T11:09:29","guid":{"rendered":"https:\/\/www.ginefiv.com\/treatment\/fish-espermatozoides\/"},"modified":"2024-09-16T05:47:13","modified_gmt":"2024-09-16T05:47:13","slug":"fish-spermatozoides","status":"publish","type":"treatment","link":"https:\/\/www.ginefiv.com\/fr\/pma\/fish-spermatozoides\/","title":{"rendered":"Analyse FISH des spermatozo\u00efdes"},"content":{"rendered":"<section class=\"page-cover treatment-section has-thumbnail dark-theme\">     <div class=\"page-cover__container container treatment-page\">         <div class=\"row\">             <div class=\"col-12 col-lg-6 pt-4 pt-lg-0\">                 <div class=\"page-cover__heading\">                     <div class=\"page-cover__heading-wrapper treatment-heading\">                                                   <div class=\"page-cover__heading-subtitle heading-subtitle text-xxl\">                             <p>PMA<\/p>                         <\/div>                          <div class=\"page-cover__heading-title heading-title title-display\">                             <h1 class=\"title-display\">Analyse FISH des spermatozo\u00efdes<\/h1>                         <\/div>                                                      <div class=\"page-cover__heading-content text-lg\">                                 <p>L\u2019hybridation fluorescente in situ (FISH) est une technique qui a r\u00e9volutionn\u00e9 la cytog\u00e9n\u00e9tique classique \u00e0 travers l&rsquo;application de connaissances issues de la biologie mol\u00e9culaire.<\/p>                             <\/div>                                                                      <\/div>                 <\/div>             <\/div>              <div class=\"col-12 col-lg-6 pt-5 pt-lg-0 pl-lg-5\">                 <div class=\"treatment-links-container\">                                     <\/div>             <\/div>         <\/div>     <\/div>               <div class=\"parallax-trigger fullsize page-cover-thumbnail treatment-thumbnail\" data-magic=\"parallax\">             <picture class=\"page-cover__picture parallax-trigger__item\" data-item=\"parallax-item\">                 <img decoding=\"async\" src=\"https:\/\/www.ginefiv.com\/wp-content\/uploads\/2023\/10\/fish-espermatozoides-header.jpg\" alt=\"Analyse FISH des spermatozo\u00efdes\">             <\/picture>         <\/div>       <\/section>   <section class=\"wp-block-breadcrumbs\">   <div class=\"content-wrapper\">     <ul>                   <li>               <a href=\"https:\/\/www.ginefiv.com\/fr\/\" title=\"Ginefiv Espa\u00f1a\">                 Ginefiv              <\/a>             <\/li>                         <li>               <span class=\"current\" title=\"Analyse FISH des spermatozo\u00efdes\">                 Analyse FISH des spermatozo\u00efdes              <\/span>             <\/li>                 <\/ul>   <\/div> <\/section>    <div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\"> <div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"> <p>La m\u00e9thode FISH vise \u00e0 \u00e9tudier le mat\u00e9riel g\u00e9n\u00e9tique des gam\u00e8tes masculines en utilisant des sondes fluorescentes qui s\u2019unissent sp\u00e9cifiquement \u00e0 un chromosome d\u00e9termin\u00e9. Il est donc possible de visualiser le nombre de chromosomes contenus dans chacun des spermatozo\u00efdes de l\u2019\u00e9jaculat.<\/p>    <p>Le recours \u00e0 la technique FISH pour analyser l\u2019\u00e9jaculat permet de d\u00e9terminer la proportion de spermatozo\u00efdes pourvus d\u2019une constitution chromosomique anormale, afin d\u2019\u00e9valuer si cette proportion est acceptable ou non.<\/p> <\/div> <\/div>   <section  class=\"image-text-block custom-block-spacing align-left\" data-magic=\"float-zigzag\">   <div class=\"container\">         <div class=\"row\">       <div class=\"img-col col-12 col-md-6\">                   <picture class=\"image-text-block__img-wrapper image-wrapper zigzag-element\">                         <img data-src=\"https:\/\/www.ginefiv.com\/wp-content\/uploads\/2023\/10\/cromosomas-1-540x380.jpg\" alt=\"\">           <\/picture>               <\/div>       <div class=\"text-col col-12 col-md-6\">         <div class=\"image-text-block__text-wrapper text-heading-block zigzag-element\">           <div class=\"image-text-block__text-wrapper__content\">             <div class=\"text-block-wrapper\">                             <h3 class=\"title-display\">L&rsquo;\u00e9tude des chromosomes<\/h3>               <p>N\u2019importe quelle cellule somatique du corps humain poss\u00e8de 23 paires de chromosomes, c\u2019est-\u00e0-dire deux copies de chaque chromosome. En revanche, pour \u00eatre g\u00e9n\u00e9tiquement normaux, les gam\u00e8tes humains (spermatozo\u00efdes et ovocytes) doivent pr\u00e9senter 23 chromosomes, c\u2019est-\u00e0-dire une unique copie de chaque chromosome. <\/p> <p>Pour pouvoir r\u00e9duire de moiti\u00e9 leur constitution chromosomique, un proc\u00e9d\u00e9 complexe de division cellulaire, appel\u00e9 m\u00e9iose, est n\u00e9cessaire. Si ce proc\u00e9d\u00e9 ne se fait pas correctement, les chromosomes ne sont pas r\u00e9partis de mani\u00e8re \u00e9gale et des gam\u00e8tes avec des anomalies chromosomiques se forment.<\/p> <p>L\u2019analyse FISH des spermatozo\u00efdes porte g\u00e9n\u00e9ralement sur cinq chromosomes : les chromosomes sexuels (X et Y) et les chromosomes 13, 18 et 21. Cette \u00e9tude peut \u00eatre \u00e9largie \u00e0 d\u2019autres chromosomes si le cas clinique le recommande. Le nombre de spermatozo\u00efdes analys\u00e9s tourne autour de 2000 en moyenne.<\/p>             <\/div>                       <\/div>         <\/div>       <\/div>     <\/div>   <\/div>  <\/section>  <section  class=\"text-block background-light custom-block-spacing\">   <div class=\"container\">     <div class=\"row\">       <div class=\"col-12\">         <div class=\"text-block__text-wrapper text-heading-block alignment-left\" data-magic=\"appear-in\">                     <h3 class=\"title-display\">Dans quels cas cette technique est-elle pr\u00e9conis\u00e9e ?<\/h3> <p>Plusieurs \u00e9tudes ont \u00e9tabli un lien de cause \u00e0 effet entre une faible qualit\u00e9 s\u00e9minale et un pourcentage \u00e9lev\u00e9 d\u2019anomalies chromosomiques dans les spermatozo\u00efdes. C\u2019est pourquoi le recours \u00e0 la m\u00e9thode FISH est recommand\u00e9e chez les patients qui ont un faible nombre de spermatozo\u00efdes (oligo ou t\u00e9ratozoospermie s\u00e9v\u00e8re). Toutefois, le principal inconv\u00e9nient dans ces cas est pr\u00e9cis\u00e9ment la difficult\u00e9 de mener ce test \u00e0 bien, \u00e9tant donn\u00e9 la faible concentration de spermatozo\u00efdes.<\/p> <p>La m\u00e9thode FISH est \u00e9galement indiqu\u00e9e dans les cas suivants :<\/p> <ul> <li>\u00c9checs d\u2019implantation.<\/li> <li>Fausses couches \u00e0 r\u00e9p\u00e9tition.<\/li> <li>Maladie chromosomique dans une grossesse pr\u00e9c\u00e9dente.<\/li> <li>Patients soumis \u00e0 chimio ou radioth\u00e9rapie.<\/li> <\/ul> <p>Les r\u00e9sultats de l\u2019analyse faciliteront le choix des professionnels quant \u00e0 la technique et au <a href=\"https:\/\/www.ginefiv.com\/fr\/pma\/\" style=\"font-weight: bold; text-decoration: underline;\" rel=\"noopener\">traitement de PMA<\/a> \u00e0 privil\u00e9gier dans chaque cas.<\/p>                   <\/div>       <\/div>     <\/div>   <\/div>  <\/section>   <div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\"> <div class=\"wp-block-column wp-column-is-centred is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:610px\" id=\"votre\"> <h3 class=\"wp-block-heading has-text-align-center\">Votre premi\u00e8re visite<\/h3>    <p class=\"has-text-align-center\">Vous avez la possibilit\u00e9 de r\u00e9aliser votre prem\u00e8re consultation sur place \u00e0 la clinique ou par vid\u00e9oconf\u00e9rence. Choisissez l\u2019option qui vous convient le mieux.<\/p>    <div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-a89b3969 wp-block-buttons-is-layout-flex\"> <div class=\"wp-block-button\"><a class=\"wp-block-button__link has-orange-background-color has-background wp-element-button\" href=\"https:\/\/www.ginefiv.com\/fr\/contact\/\" style=\"border-radius:5px\">Contactez-nous<\/a><\/div> <\/div> <\/div> <\/div>    <div class=\"wp-block-columns alignfull has-turquoise-teal-background-color has-background is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\"> <div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"> <section class=\"grid-icons-block content-wrapper\">        <div class=\"grid-icon\">             <picture class=\"grid-icon__thumbnail\">         <img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"700\" src=\"https:\/\/www.ginefiv.com\/wp-content\/uploads\/2023\/10\/35anos.png\" class=\"attachment-large size-large\" alt=\"\" srcset=\"https:\/\/www.ginefiv.com\/wp-content\/uploads\/2023\/10\/35anos.png 700w, https:\/\/www.ginefiv.com\/wp-content\/uploads\/2023\/10\/35anos-300x300.png 300w, https:\/\/www.ginefiv.com\/wp-content\/uploads\/2023\/10\/35anos-150x150.png 150w, https:\/\/www.ginefiv.com\/wp-content\/uploads\/2023\/10\/35anos-420x420.png 420w\" sizes=\"auto, (max-width: 700px) 100vw, 700px\" \/>      <\/picture>             <div class=\"grid-icon__text\">         35 ans d&rsquo;exp\u00e9rience      <\/div>     <\/div>         <div class=\"grid-icon\">             <picture class=\"grid-icon__thumbnail\">         <img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"700\" src=\"https:\/\/www.ginefiv.com\/wp-content\/uploads\/2023\/10\/mano-cruz.png\" class=\"attachment-large size-large\" alt=\"\" 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